Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora)
Autor: | Shi-Chao Xing, Changwei Shao, Yongsheng Tian, Song-Lin Chen, Xiaolin Liao |
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Rok vydání: | 2008 |
Předmět: | |
Zdroj: | Conservation Genetics. 10:789-791 |
ISSN: | 1572-9737 1566-0621 |
DOI: | 10.1007/s10592-008-9663-5 |
Popis: | In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909 to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result, 13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in Nibea albiflora. |
Databáze: | OpenAIRE |
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