Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora)

Autor: Shi-Chao Xing, Changwei Shao, Yongsheng Tian, Song-Lin Chen, Xiaolin Liao
Rok vydání: 2008
Předmět:
Zdroj: Conservation Genetics. 10:789-791
ISSN: 1572-9737
1566-0621
DOI: 10.1007/s10592-008-9663-5
Popis: In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909 to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result, 13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in Nibea albiflora.
Databáze: OpenAIRE