Quantitation and occurrence of hydroxy fatty acids in fats and oils

Autor:	Daniel P. Schwartz, Aly H. Rady
Rok vydání:	1992
Předmět:	Chromatography genetic structures Cyclohexane Chemistry General Chemical Engineering Glyceride Organic Chemistry Fraction (chemistry) behavioral disciplines and activities Chloride chemistry.chemical_compound mental disorders medicine Organic chemistry Pyruvic acid medicine.drug
Zdroj:	Journal of the American Oil Chemists’ Society. 69:170-173
ISSN:	1558-9331 0003-021X
DOI:	10.1007/bf02540570
Popis:	A relatively simple method is detailed for the routine quantitation and isolation of hydroxy fatty acids (HFA) in the glycerides of lipids. The lipid in cyclohexane is transmethylated in a two-phase, 3.5 min procedure, and the hydroxyl compounds in the methyl ester fraction are derivatized with pyruvic acid chloride 2,6-dinitrophenyl-hydrazone in the presence of triethylenediamine. The derivatives are fractionated on alumina, and the HFA fraction is evaluated spectrophotometrically. A large variety of animal, plant and marine lipids contain HFA in concentrations ranging from 1000 μmoles/g lipid. The derivatives lend themselves admirably to purification techniques. A procedure for regenerating the parent HFA from the derivative is described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0a4d6aa8bc205fa2e4d6f2ab578d8bb7 https://doi.org/10.1007/bf02540570 Zobrazit plný text záznamu Full text from SpringerLink Plný text