Mechanisms of interaction of Cetylpyridinium chloride with Staphylococcus aureus in the presence of β-cyclodextrin
| Autor: | Alan R. de Oliveira, Jeferson G. Da Silva, Larissa M. D. Andrade, Guilherme F. da Silva, Gabriella Freitas Ferreira, Thiago M. de Miranda, Ângelo M. L. Denadai |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Cyclodextrin 010405 organic chemistry technology industry and agriculture Isothermal titration calorimetry macromolecular substances General Chemistry 010402 general chemistry Condensed Matter Physics medicine.disease_cause Antimicrobial Cetylpyridinium chloride 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry Dynamic light scattering Pulmonary surfactant Staphylococcus aureus Zeta potential medicine Food Science Nuclear chemistry |
| Zdroj: | Journal of Inclusion Phenomena and Macrocyclic Chemistry. 97:205-215 |
| ISSN: | 1573-1111 1388-3127 |
| Popis: | Cetylpyridinium chloride (CPC) is a cationic surfactant, which has a biocidal activity against a broad spectrum of bacteria, including Staphylococcus aureus. This microorganism, although frequently found in the normal human microbiota, can become a pathogen that causes a wide variety of infections. Thus, the present work aims to investigate the effect of the β-cyclodextrin (βCD) on CPC antimicrobial activity against S. aureus, especially in the mechanism of interaction with S. aureus membrane. For these purposes, in vitro antimicrobial susceptibility of CPC and CPC/βCD compounds against S. aureus were determined by calculating the lower concentration capable of reducing cell viability by 50 and 100 percent, and the kinetics of bacterial death were evaluated by kill curves for the two systems. After that, the colloidal mechanisms of interaction of the CPC and CPC/βCD against S. aureus were investigated by Dynamic Light Scattering (DLS) and Zeta Potential (ZP) titrations. Finally, the thermodynamic parameters of drug-cell interaction were determined by Isothermal Titration Calorimetry (ITC), in order to obtain deeper understanding of the mechanism of drug interactions. The results of DLS, ZP and ITC showed that in presence of βCD occurs a different mechanism of interaction with S. aureus membrane, explaining therefore the higher antimicrobial activity of CPC/βCD system. |
| Databáze: | OpenAIRE |
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