Liquid chromatography of tylosin A and related substances on poly(styrene-divinylbenzene)

Autor:	P Claeys, Jos Hoogmartens, W Cypers, J Paesen, Eugene Roets
Rok vydání:	1995
Předmět:	Chromatography Organic Chemistry General Medicine Tylosin Divinylbenzene Biochemistry Analytical Chemistry Styrene chemistry.chemical_compound chemistry Impurity Potassium phosphate Phase (matter) Selectivity Quantitative analysis (chemistry)
Zdroj:	Journal of Chromatography A. 699:93-97
ISSN:	0021-9673
DOI:	10.1016/0021-9673(94)01268-j
Popis:	An isocratic liquid chromatography method for assay and purity control of erythromycin is presented. Erythromycin A is separated from all its potential impurities, except erythromycin D. The selectivity depends on the pore size of the poly(styrene-divinylbenzene) stationary phase. Wide pore PLRP-S 8 μm 1000 A shows the best selectivity. The column is heated at 70°C. The mobile phase is acetonitrile-2-methyl-2-propanol-0.2 M potassium phosphate buffer pH 9.0-water (3:16.5:5:75.5). The flow rate is 2 ml/min. UV detection is performed at 215 nm. The total analysis time is about 30 min. The method was used to compare official standards.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0a3b21b2c294d913a957f85c064d2345 https://doi.org/10.1016/0021-9673(94)01268-j Zobrazit plný text záznamu Full Text from ScienceDirect