| Autor: |
George G. Bierkamper |
| Rok vydání: |
1981 |
| Předmět: |
|
| Zdroj: |
European Journal of Pharmacology. 73:343-348 |
| ISSN: |
0014-2999 |
| DOI: |
10.1016/0014-2999(81)90235-1 |
| Popis: |
The effects of the anticholinesterase agent, diisopropyfluorophosphate (DFP) on neuromuscular transmission were explored using intracellular recording techniques in a vascular perfused rat phrenic nerve-hemidiaphragm preparation. This study was designed to assess the applicability of DFP when used to inhibit acetylcholinesterase for the purpose of recovering released acethylcholine. A procedure was used in which a single cell was held before and after drug. The presence of DFP (4×10 −5 M) in the perfusion medium yielded greater than 97% inhibition of acetylcholinesterase activity and resulted in a slight increase in amplitude as well as a prolongation of the decay phase of spontaneous miniature endplate potentials and evoked endplate potentials. Quantal content of evoked acetylcholine release was unaltered by DFP. The prolonged decay phase and increased amplitude remained after washout of unbound DFP suggesting an effect through irreversible binding. In the absence of change in quantal content it is concluded that DFP (4×10 −5 M) has no significant effect on neurotransmitter release. Therefore, DFP appears to be an appropriate acetylcholinesterase inhibitor for studies involving the release of endogenous acetylcholine from phrenic nerve-hemidiaphragm. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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