| Popis: |
SUMMARY We aimed to examine the distribution of SEPT4, SEPT5, and SEPT8 in the hu- man eye. For each septin, five to six normal human eyes were examined by immunohis- tochemical staining of paraffin sections using polyclonal antibodies against SEPT4, SEPT5, and SEPT8 and an avidin biotin complex immunodetection system. SEPT4 immunoreactivity (IR) was detected primarily in the epithelium of cornea, lens, and nonpigmented ciliary ep- ithelium; in the endothelium of cornea and vessels of iris and retina; and in the retinal nerve fiber layer, the outer plexiform layer, the outer segments of the photoreceptor cells, the inner limiting membrane of the optic nerve head, and optic nerve axons. SEPT5-IR was present in corneal endothelial cells, iris tissue, nonpigmented ciliary epithelium, and epi- thelial cells of the lens. SEPT8-IR almost paralleled that of SEPT4, except for a lower SEPT8-IR of the outer photoreceptor segments and a positive staining of the meningothelial cell nests in the subarachnoidal space of the bulbar segment of the orbital optic nerve. In con- clusion, SEPT4, SEPT5, and SEPT8 are expressed in various ocular tissues, each revealing a dis- tinct expression pattern. Both physiological and potential pathophysiological role of sep- tins in the human eye deserve further investigation. (J Histochem Cytochem 53:1139-1147, 2005) |
