A Basic Method for Hoechst (33258) Staining of Nuclei from Whole Root Tissues of Oryza sativa
| Autor: | Snehalata Majumdar, Rita Kundu, Falguni Barman, Titir Guha |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Oryza sativa Chemistry 02 engineering and technology Protoplast 01 natural sciences Fluorescence Staining Cell wall Nuclear morphology Hoechst stain chemistry.chemical_compound medicine.anatomical_structure 0202 electrical engineering electronic engineering information engineering Biophysics medicine 020201 artificial intelligence & image processing Engineering (miscellaneous) Nucleus 010606 plant biology & botany |
| Zdroj: | National Academy Science Letters. 43:389-392 |
| ISSN: | 2250-1754 0250-541X |
| DOI: | 10.1007/s40009-019-00865-3 |
| Popis: | Nuclear morphology studies are a reliable parameter for analysis of genotoxic properties of different agents. Such studies have been widely exploited in isolated protoplasts but seldom used in cells isolated from whole root tissue due to cell wall permeability restrictions. We present a rapid and easy protocol that will benefit the researchers in studying the nucleus stained with fluorescent dye. Here, we have modified the digestion buffer and used a permeabilization buffer to ease the entry of the fluorescent Hoechst stain through the cell wall, so that fluorescence can be observed under UV light. |
| Databáze: | OpenAIRE |
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