Construction of Full-Length Infectious Clones of Turnip Mosaic Virus Isolates Infecting Perilla Frutescens and Genetic Analysis of Recently Emerged Strains of Tumv in Korea

Autor: Jin Sung Hong, Zheng-Xing Song, Tae-Seon Park, Wen-Xing Hu, John Hammond, Eun-Young Seo, In-Sook Cho, Su-Jeong Chu, Ji-Soo Park, Hyoun-Sub Lim, Yong Pyo Lim
Rok vydání: 2021
Předmět:
Popis: Perilla is an annual herb with a unique aroma and taste and has been cultivated in Korea for hundreds of years. Owing to the highly edible and medicinal value of Perilla plants, it has been widely cultivated in many Asian and European countries. Recently, several viruses have been reported to cause diseases in Perilla in Korea, including turnip mosaic virus (TuMV) which is known as a brassica pathogen due to its significant damage to brassica crops. In this study, we determined the complete genome sequences of two new TuMV isolates originating from Perilla in Korea. Full-length infectious cDNA clones of these two isolates were constructed and their infectivity was tested by agroinfiltration on Nicotiana benthamiana and sap inoculation on Chinese cabbage and radish. In addition, we analyzed the phylogenetic relationship of six new Korean TuMV isolates and determined their respective affiliation with the four major groups. We also conducted recombination analysis for isolates recently occurring in Korean using RDP4 software, which provided new insight into the evolutionary relationships among Korean isolates of TuMV.
Databáze: OpenAIRE