Influence of introducing the genetically modified strain Sinorhizobium meliloti ACH-5 on the structure of the soil microbial community

Autor:	A. G. Pinaev, E. V. Korostik, S. N. Petrova, G. A. Akhtemova, E. P. Chizhevskaya, E. E. Andronov
Rok vydání:	2009
Předmět:	Sinorhizobium meliloti Genetically modified microorganisms Strain (biology) Microorganism Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Genetically modified organism Microbial population biology Botany Bacteria Archaea
Zdroj:	Microbiology. 78:474-482
ISSN:	1608-3237 0026-2617
DOI:	10.1134/s0026261709040110
Popis:	To study the influence of genetically modified microorganisms (GMM) on the number and structure of the soil microbial community, we introduced the genetically modified strain of Sinorhizobium meliloti into soil under controlled laboratory conditions. The analysis of the dynamics of soil microorganisms of all the main groups (archaea, bacteria, fungi) using the PCR with real-time detection and the analysis of the species structure of all the indicated components using T-RFLP were carried out for a month. The results of the quantitative PCR demonstrated that none of the components of the soil microbial community was appreciably influenced by the GMM introduced. The number of GMM decreased over a month more than 300-fold. The analysis of the dynamics of the eubacterial, archaeal, and fungal communities using T-RFLP did not detect fundamental changes in their structure.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::09f0f81cbfbd820a40babd0b477f97c5 https://doi.org/10.1134/s0026261709040110 Zobrazit plný text záznamu Full text from SpringerLink