Combination of Developmental Behaviors and Transcriptome Reveals Differential Response Mechanisms of Phytophthora sojae to Aspartic Acid and Glucose in Seed Exudates
| Autor: | Yifan Zhao, Haixu Liu, Zhuoqun Zhang, Tai An, Jingzhi Wen, Xinying Gao, Xiangqi Bi, Han Yu |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
chemistry.chemical_classification
Cell signaling biology medicine.diagnostic_test Zoospore Proteolysis Chemotaxis Plant Science biology.organism_classification Amino acid Transcriptome chemistry.chemical_compound chemistry Biochemistry medicine Phytophthora sojae Phosphatidylinositol Agronomy and Crop Science |
| Zdroj: | Phytopathology®. 112:620-629 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-08-21-0332-r |
| Popis: | Isoflavones in soybean seed and root exudates are host-specific signal molecules for Phytophthora sojae to recognize its host, soybean. G protein and calcium signaling pathways are involved in the chemotaxis of zoospores in the recognition of isoflavones. The role played by non-host-specific signaling molecules (sugars and amino acids) found in seed and root exudates on zoospore chemotaxis and mycelial growth was studied. The transcriptome of P. sojae responding to aspartic acid (Asp) and glucose (Glc) was analyzed in parellel by the RNA-seq method. We found that the relative in situ concentrations of amino acids and sugars significantly promoted zoospore chemotaxis, as do isoflavones. Transcriptomics showed that both similarities and differences existed in response mechanisms of P. sojae to Asp and Glc. Asp and Glc activated mitogen-activated protein kinase signaling pathways and the phosphatidylinositol signaling system but not the G-protein signaling pathway, which have been reported to be responsible for zoospore chemotaxis. In addition, ubiquitin-mediated proteolysis and ATP binding cassette transporters were also activated by Asp and Glc. Meanwhile, the glutathione signaling pathway uniquely participated in the response of P. sojae to Asp but was not involved in the response process to Glc, which will be evaluated in another study. Our results provide new insights into the molecular mechanisms of zoospore response to Asp and Glc. |
| Databáze: | OpenAIRE |
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