Parameters for optimizing detection of transforming growth factors

Autor:	Eric Ruff, Angie Rizzino
Rok vydání:	1986
Předmět:	urogenital system Growth factor medicine.medical_treatment Clone (cell biology) Cell Biology Biology Bovine plasma Pathology and Forensic Medicine Cell biology Epidermal growth factor Cell culture Soft agar Immunology medicine Anatomy Anchorage-Independent Growth Transforming growth factor
Zdroj:	Journal of Tissue Culture Methods. 10:109-115
ISSN:	1573-0603 0271-8057
Popis:	Transforming growth factors (TGF) induce the soft agar growth of nontransformed cells, such as NRK-49F cells. This report describes culture conditions that optimize the detection of TGF by NRK-49F cells. Two aspects are dealt with in depth: the need to select an appropriate clone of NRK-49F cells and the need to select a culture medium that supports little or no background growth in the absence of added TGF. Evidence is presented to demonstrate that medium supplemented with heat-treated, dialyzed bovine plasma provides better conditions for assaying TGF than serum-supplemented medium provides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::09d64c5bbe6ef3b5bbc304ac50e6f8c1 https://doi.org/10.1007/bf01404601 Zobrazit plný text záznamu