Calcium phosphate transfection mammalian cells v1
| Autor: | Karla Feijs |
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| Rok vydání: | 2021 |
| Předmět: | |
| Popis: | This is our calcium phosphate transfection protocol. It always works with HEK293T cells, for other lines the transfection efficiency depends on their source: some HeLa and U2OS clones can be transfected using CaPO4, however most cannot. We mostly use this method to transfect HEK293T cells for lentivirus production. |
| Databáze: | OpenAIRE |
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