Application of the inhibition of bacterial bioluminescence test for assessment of toxicity of carbon-based nanomaterials

Autor:	Dmitrij Gennad'evich Deryabin, Elena Sergeevna Aleshina, Ljudmila Viktorovna Efremova, Dmitry Deryabin
Rok vydání:	2012
Předmět:	Materials science Bioluminescence test chemistry.chemical_element Nanotechnology Applied Microbiology and Biotechnology Microbiology Nanomaterials chemistry Carbon based nanomaterials Toxicity Bioluminescence Luminescence Biosensor Carbon
Zdroj:	Microbiology. 81:492-497
ISSN:	1608-3237 0026-2617
DOI:	10.1134/s0026261712040042
Popis:	A method for assessment of integral biological toxicity of carbon-based nanomaterials (CBN) using a bacterial luminescent biosensor was developed, which accounted for the properties of the objects under study. The proposed approach includes a special procedure for the preparation of the analyzed CBN samples aimed at obtaining highly dispersed suspensions, an extended period of dynamic monitoring of the luminescence of the contacting sensor microorganism, and the special algorithm for quantification of the results of the bioluminescence analysis, in order to exclude the effect of the optical properties of CBN on the results of investigation. This method was used for assessment of the biological toxicity of a broad spectrum of CBN, such as single- and multi-walled nanotubes, nanofibres, and C60- and C70-fullerenes, in order to compare them in this respect to amorphous (nonstructured) carbon.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::09ce7d8aee132b0702c64ba3788588d7 https://doi.org/10.1134/s0026261712040042 Zobrazit plný text záznamu Full text from SpringerLink