Intercellular Ca2+ Response Triggered by IP3 among Endothelial Cells in Shear Stress Flow

Autor:	Tateki Sumii, Makoto Hosobuchi, Marie Terada, Shogo Kuretoko, Tomoya Shimada, Kazuo Tanishita, Susumu Kudo
Rok vydání:	2011
Předmět:	Endothelial stem cell Paracrine signalling Chemistry Mechanical Engineering Second messenger system Gap junction Biophysics Shear stress Nanotechnology Signal transduction Condensed Matter Physics Shear flow Intracellular
Zdroj:	TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series B. 77:2431-2441
ISSN:	1884-8346 0387-5016
DOI:	10.1299/kikaib.77.2431
Popis:	A phenomenon has been observed in which intracellular Ca2+ concentration in endothelial cells increases upon application of shear stress (Ca2+ response). It is therefore assumed that Ca2+ is the second messenger in the transfer of shear stress stimulation into cells. The Ca2+ response is also known to spread to surrounding cells (Ca2+ wave). We investigated the effects on Ca2+ wave among cultured bovine aorta endothelial cells (BAECs) upon inhibiting the main intercellular signaling pathways, such as gap junction and paracrine pathways by inducing Ca2+ wave using D-myo-inositol 1,4,5-trisphosphate, P4(5)-(1-(2-nitrophenyl)ethyl) ester trisodium salt (Caged IP3) due to an intracellular IP3 elevation. In addition, we investigated the Ca2+ wave among BAECs under shear stress loading. Using Caged IP3, local release of ATP from BAEC induced Ca2+ wave. The Ca2+ wave was inhibited by the inhibitors of paracrine pathways. Furthermore, the Ca2+ response spread in the direction of the downstream under shear flow. These results suggest that paracrine pathway is dominant in both of flow and no flow conditions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::09c22da6b0d7a53911e9df8a8217c5dc https://doi.org/10.1299/kikaib.77.2431 Zobrazit plný text záznamu