Genetic Profile Evaluation of Human Cell Lines Treated with Anastatica hierochuntica Using Forensic DNA Fingerprinting Markers
| Autor: | Saranya Rameshbabu, Mohammed S. Ali, Abrar B. Alsaleh, Anuradha Venkatraman, Safia A. Messaoudi |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Arab Journal of Forensic Sciences and Forensic Medicine. 3:231-244 |
| ISSN: | 1658-6794 1658-6786 |
| DOI: | 10.26735/fcoi3663 |
| Popis: | Cell line authentication using Short Tandem Repeats (STRs) is necessary to ensure the integrity of the cell for its continuous culture and to identify misidentification and cross-contamination issues. This study investigates the changes in the genetic profile of MCF-7 and HepG2 cell lines caused by the methanolic leaf extract of Anastatica hierochuntica (AH) using human identification based STR markers. MCF-7 and HepG2 cell lines were treated with various concentrations of AH extracts for three different periods. The treated and control cells' DNA was extracted using a QIAamp® DNA Micro Kit, quantified using a Quantifiler Duo DNA Quantification Kit, and amplified using an AmpFlSTR Identifiler plus PCR Amplification Kit. The concentrations of the DNA extracted from control and MCF-7 and HepG2 cell lines treated with AH extract at 300 to 2400 µg/ml for 24hr and 150 to 2400 µg/ml for 48 and 72hrs were statistically significant (p |
| Databáze: | OpenAIRE |
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