Fully hydrated and highly oriented membranes: an experimental setup amenable to specular and diffuse X-ray scattering
| Autor: | Tim Salditt, Dominique Thiaudière, Christian Münster, M. Vogel, W. Fenzl |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Materials science
Silicon business.industry Scattering Bilayer Resolution (electron density) X-ray chemistry.chemical_element Condensed Matter Physics Electronic Optical and Magnetic Materials Optics Membrane chemistry Chemical physics Sample preparation Specular reflection Electrical and Electronic Engineering business |
| Zdroj: | Physica B: Condensed Matter. 283:32-36 |
| ISSN: | 0921-4526 |
| DOI: | 10.1016/s0921-4526(99)01886-4 |
| Popis: | We describe an experimental setup and sample preparation procedure, which makes it possible to study highly aligned phospholipid membranes under conditions of full hydration, i.e. in excess water. While full hydration is an essential requirement to determine biologically relevant bilayer structure and fluctuation properties, the high degree of orientation with respect to the silicon or glass substrates (mosaicities below the instrumental resolution) allows for the application of modern interface sensitive scattering techniques like specular reflectivity and nonspecular (diffuse) X-ray scattering. Furthermore, multilamellar stacks of membranes with intercalated water can be prepared to increase the scattering signal and to get information on intermembrane potentials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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