Development of an Artificial Placenta: Endocrine Responses of Goat Fetuses during Long-Term Extrauterine Incubation with Umbilical Arteriovenous Extracorporeal Membrane Oxygenation
| Autor: | Kimihiro Takechi, Haruo Masuda, Yuji Taketani, Yoshiko Ogami, Nobuya Unno, Shiro Kozuma, Masato Sakai, Yoshinori Kuwabara, Takashi Okai, Ruriko Tsushima, Yumiko Narumiya-Takikawa, Kazunori Baba |
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| Rok vydání: | 1994 |
| Předmět: |
Fetus
medicine.medical_specialty Amniotic fluid Endocrinology Diabetes and Metabolism medicine.medical_treatment Adrenocorticotropic hormone Biology Endocrinology medicine.anatomical_structure In utero Internal medicine Placenta Circulatory system medicine Extracorporeal membrane oxygenation Incubation |
| Zdroj: | Endocrine Journal. 41:S69-S76 |
| ISSN: | 1348-4540 0918-8959 |
| DOI: | 10.1507/endocrj.41.supplement_s69 |
| Popis: | To investigate endocrine responses of isolated premature goat fetuses during long-term extrauterine incubation with umbilical arteriovenous extracorporeal membrane oxygenation (A-V ECMO), we conducted experiments in seven goat fetuses (95-134 days gestation). The fetuses were cannulated from the umbilical vessels, and their blood-gas exchange was totally supported by A-V ECMO, while they were maintained in an isothermal incubator containing artificial amniotic fluid. The survival period was between 84 and 190 h. At 24-h intervals, fetal blood samples were collected, and plasma concentrations of catecholamines, ACTH, and cortisol were determined. After 24 h of incubation, fetal circulatory and respiratory variables remained stable, until evident circulatory failure occurred before their deaths. A similar pattern was observed in temporal changes in plasma concentrations of catecholamines, ACTH, and cortisol. Plasma levels were high during the initial 24 h of incubation, subsequently decreased, and then increased before death. Hormone levels during stable periods were equivalent to or slightly higher than values for fetuses in utero. These results suggest that conditions during the stable period of long-term extracorporeal fetal incubation are not highly stressful for the isolated fetuses. |
| Databáze: | OpenAIRE |
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