Lab-on-a-chip enabled HLA diagnostic: combined sample preparation and real time PCR for HLA-B57 diagnosis
| Autor: | Rainer Frank, Andreas Willems, Christian Moche, Nadine Hlawatsch, Richard Klemm, Holger Becker, Sebastian Schattschneider, Claudia Gärtner |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Autoimmune disease
Lysis Computer science Microfluidics Antigen presentation Human immunodeficiency virus (HIV) Computational biology Human leukocyte antigen Lab-on-a-chip medicine.disease Bioinformatics medicine.disease_cause DNA extraction law.invention Autoimmunity Blood cell medicine.anatomical_structure Immune system Real-time polymerase chain reaction law Abacavir medicine Sample preparation medicine.drug |
| Zdroj: | Advances in Global Health through Sensing Technologies 2015. |
| ISSN: | 0277-786X |
| DOI: | 10.1117/12.2178897 |
| Popis: | The diverse human HLA (human leukocyte antigen) system is responsible for antigen presentation and recognition. It is essential for the immune system to maintain a stable defense line, but also is also involved in autoimmunity as well as metabolic disease. HLA-haplotype (HLA-B27), for instance, is associated with inflammatory diseases such as Bechterew's disease. The administration of the HIV drug Abacavir in combination with another HLA-haplotype (HLAB57) is associated with severe hypersensitivity reactions. Accordingly, the HLA status has to be monitored for diagnosis or prior to start of therapy. Along this line, a miniaturized microfluidic platform has been developed allowing performing the complete analytical process from “sample-in” to “answer-out” in a point-of-care environment. The main steps of the analytical cascade inside the integrated system are blood cell lysis and DNA isolation, DNA purification, real-time PCR and quantitative monitoring of the rise of a fluorescent signal appearing during the PCR based sequence amplification. All bio-analytical steps were intended to be performed inside one chip and will be actuated, controlled and monitored by a matching device. This report will show that all required processes are established and tested and all device components work well and interact with the functional modules on the chips in a harmonized fashion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|