| Autor: |
Stefanie Martin, T. Steck, Ursula Zollner, Jürgen Liebermann |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Acta Obstetricia et Gynecologica Scandinavica. 78:326-331 |
| ISSN: |
0001-6349 |
| DOI: |
10.1034/j.1600-0412.1999.780410.x |
| Popis: |
Background. The aim of this study was to enhance the predictability of conventional semen parameters for in-vitro fertilization outcome. The utility of late sperm motility in presence of a cumulus-oocyte complex after different hours of incubation was investigated to predict the outcome of IVF in borderline and normal ejaculates (at least 5×10 6 motile sperm). Methods. The study was done on 52 infertile couples undergoing conventional in-vitro fertilization and embryo transfer. Sperm were prepared by the Percoll cushion centrifugation with swim-down. Cocultures were established by inseminating the cumulus-oocyte complexes with 100 000 motile spermatozoa and incubating them for 48 hours. Another 100 000 spermatozoa were incubated in culture medium for 48 hours. Sperm motility (WHO a+b) was determined at 0, 4, 24 and 48 hours of incubation. Results. The fertilization rate was 65.5% (42.9-88.1). The conventional semen parameters did not correlate with the fertilization rate. Sperm motility measured after different hours of incubation was found to be significantly positively correlated with the fertilizing ability of sperm in vitro in Spearman's rank correlation test: motility after 0 h (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
