Identification of heat tolerance loci in broccoli through bulked segregant analysis using whole genome resequencing
| Autor: | Mark W. Farnham, Sandra E. Branham |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics education.field_of_study Population Bulked segregant analysis food and beverages Single-nucleotide polymorphism Plant Science Horticulture Marker-assisted selection Biology Quantitative trait locus 01 natural sciences Genome 03 medical and health sciences 030104 developmental biology Genetic marker Doubled haploidy education Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Euphytica. 215 |
| ISSN: | 1573-5060 0014-2336 |
| DOI: | 10.1007/s10681-018-2334-9 |
| Popis: | Most broccoli cultivars are sensitive to high temperatures during the early stages of floral development causing a severe decline of head quality or even complete lack of head formation under superoptimal crop production temperatures. Several heat tolerant lines have been developed in recent years but there have been few studies of the genetic basis of this complex, polygenic trait. A doubled haploid population of broccoli was evaluated for head quality across two summer field trials with the phenotypic extremes validated in two additional summer fields. Whole-genome resequencing of the bulked segregants was used for a quantitative trait loci (QTL)-seq analysis of heat tolerance. Two novel QTL, which differ from previously reported QTL, were identified. Nonsynonymous SNPs were found in a block of flowering time genes within QHT_C09.2 and may explain the significant negative correlation between time to head maturity and heat tolerance. Breeding further genetic gains in this complex, polygenic trait could be expedited through marker assisted selection and gene pyramiding using markers developed from the QTL identified herein. |
| Databáze: | OpenAIRE |
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