Use of different anticoagulants in test tubes for analysis of blood lactate concentrations
| Autor: | Bart Chernow, Roger Kerzner, Jeff Wiese, Aarati Didwania |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Chromatography business.industry medicine.drug_class Anticoagulant Venous blood Heparin Critical Care and Intensive Care Medicine Intensive care unit Surgery law.invention chemistry.chemical_compound chemistry law Sodium citrate Blood lactate Medicine Hyperlactatemia business Test tube medicine.drug |
| Zdroj: | Critical Care Medicine. 25:1847-1850 |
| ISSN: | 0090-3493 |
| Popis: | Objectives a) To test the hypothesis that the measurement of the circulating lactate concentration is influenced by the anticoagulant in the test tube that contains the blood sample; b) to test the hypothesis that the measurement of the circulating lactate concentration is influenced by the tissue used for analysis. Design A prospective, controlled study. Setting A critical care research laboratory, a 20-bed intensive care unit (ICU), and the general wards. Subjects Twenty-three ICU and ward patients with hyperlactatemia and 19 healthy volunteers. Interventions Blood samples were collected for determination of blood lactate concentration. Measurements and main results Venous blood samples (12 mL) were obtained from each of the 19 normal subjects and each 12-mL specimen was evenly divided into six aliquot portions (six test tubes). Experiment 1: Of the six tubes, two tubes were set aside for experiment 2. The other four tubes were used to test four anticoagulants (one anticoagulant per tube). The anticoagulants tested were: sodium heparin; EDTA; lithium heparin; and sodium citrate. Lactate concentrations were analyzed using an ion-selective, amperometric electrode that we have previously validated. There were no statistically significant differences between the lactate concentrations derived from blood samples stored in sodium heparin, EDTA, or lithium heparin (p > .05; n = 19; Student-Newman-Keuls' multiple comparisons test). The lactate concentration of blood stored in sodium citrate, however, was lower than all other anticoagulants (p 2.2 mmol/L, there was a difference of 0.14 mmol/L (p .05; Student-Newman-Keuls' test). Conclusions a) Sodium citrate, as an anticoagulant, caused lower lactate concentrations to be measured as compared with heparin or EDTA; b) the measurement of lactate concentrations in plasma or serum samples yields a higher value than the concentration found in the original whole blood specimen. |
| Databáze: | OpenAIRE |
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