Hydrolase activity as an indicator of the state of microbial communities in vermicomposts

Autor:	B. A. Byzov, A. V. Yakushev
Rok vydání:	2009
Předmět:	chemistry.chemical_classification biology fungi Hydrolase activity Biomass General Medicine engineering.material biology.organism_classification Manure Enzyme assay Hydrolysis Enzyme Agronomy chemistry biology.protein engineering Food science Vermicompost Bacteria
Zdroj:	Moscow University Soil Science Bulletin. 64:93-98
ISSN:	1934-7928 0147-6874
Popis:	The hydrolase activity was studied using the fluorescein diacetate (FDA) hydrolysis assay in order to assess the microbial activity in composts and vermicomposts obtained with participation of Eisenia fetida andrei, Aporrectodea caliginosa, and A. rosea earthworms from cattle manure, leaves, and peat. The hydrolase activity (the Michaelis—Menten constant, K m ) was found to be lower in the vermicomposts than in the composts and correlated with a corresponding decrease in the length of fungal hyphae. The determination of the hydrolase activity according to the reaction of FDA hydrolysis may be recommended to control the process of vermicomposting and the quality of vermicomposts. Thus, the process of vermicomposting may be characterized with the use of the functional (hydrolase activity) and structural (microbial biomass, length of fungal hyphae) approaches.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::095286ba1f49c7e4f6d20c3d1259e94a https://doi.org/10.3103/s0147687409020082 Zobrazit plný text záznamu Full text from SpringerLink