Expression of recombinant E1 Glycoprotein of Chikungunya virus in Baculovirus expression system
| Autor: | Magdline Sia Henry Sum, Anna Andrew, Milda Aren Maling |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Biochemistry, Microbiology and Biotechnology. 3:26-29 |
| ISSN: | 2289-5779 |
| DOI: | 10.54987/jobimb.v3i1.232 |
| Popis: | Chikungunya is an acute febrile illness caused by chikungunya virus (CHIKV). In this study, the envelope E1 gene of CHIKV was cloned and expressed in a baculovirus system. The recombinant E1 protein with N-term 6-His residues protein was successfully expressed and purified as confirmed by SDS-PAGE and western blot analysis. The seroreactivity of the recombinant protein was evaluated in immunoassay for anti-CHIKV IgM and IgG antibodies. The recombinant antigen showed 69% sensitivity and 100% specificity for anti-CHIKV IgG by dot blot assay. Detection of anti-CHIKV IgM by dot assay showed 79% sensitivity and 100% specificity. No cross reactivity of the antigen was observed with anti-dengue virus serum samples. The results strongly support that the recombinant E1 protein has potential to be used as diagnostic antigen. The used of the antigen in a dot blot assay gives an advantage for laboratory detection without the need of any specialised equipment. |
| Databáze: | OpenAIRE |
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