Interplay between hydrogen sulfide and calcium/calmodulin enhances systemic acquired acclimation and antioxidative defense against nickel toxicity in zucchini
| Autor: | Ali Mohammad Ahadi, Rayhaneh Amooaghaie, Maryam Valivand |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Antioxidant Calmodulin medicine.medical_treatment chemistry.chemical_element Hypotaurine Plant Science Calcium medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound medicine Extracellular Proline Ecology Evolution Behavior and Systematics biology equipment and supplies EGTA 030104 developmental biology chemistry Biophysics biology.protein Agronomy and Crop Science Oxidative stress 010606 plant biology & botany |
| Zdroj: | Environmental and Experimental Botany. 158:40-50 |
| ISSN: | 0098-8472 |
| DOI: | 10.1016/j.envexpbot.2018.11.006 |
| Popis: | In this study 50 mg/l Ni stress reduced plant growth, induced oxidative stress, increased proline accumulation and H2S content in roots and leaves of zucchini. Indeed, Ni toxicity up-regulated calmodulin (CaM) gene expression in leaves. Root pretreatment with calcium and NaHS (as hydrogen sulfide donor) singly or in combination reduced SOD activity but enhanced activities of CAT, POD and lowered accumulation of proline and Ni element not only in root, but also in untreated leaves, suggesting a systemic mitigating effect of Ca2+/H2S pretreatment on Ni-evoked oxidative damage in both roots and leaves. Importantly, Ni-induced H2S accumulation was markedly strengthened by application of exogenous Ca2+ and this effect was weakened by addition of H2S scavenger, hypotaurine (HT), in roots and leaves, suggesting H2S acts downstream of Ca2+. On the other hand, root pretreatment with NaHS, amplified Ni-induced CaM gene expression in leaves and mediated antioxidant defense in both roots and leaves and addition of ethylene glycol tetra acetic acid (EGTA) as a Ca2+ chelator, verapamil (VPL) as a plasma membrane channel blocker, and trifluoperazine(TFP) as a calmodulin (CaM) antagonist, reversed these impacts. These results suggested that root pretreatment with H2S could induce systemic Ni tolerance not only in the site of application, but also in untreated distant parts in Ni-stressed zucchini seedlings and the acquisition of this Ni tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM. Taken together, it seems that Ca2+/CaM complex functions both upstream and downstream of H2S and crosstalk of Ca2+ and H2S is essential for inducing systemic acquired acclimation in Ni-stressed zucchini seedlings. |
| Databáze: | OpenAIRE |
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