A Perspective on the Development of Tandem Mass Spectrometry

Autor: Christie G. Enke
Rok vydání: 2016
Předmět:
DOI: 10.1016/b978-0-08-043848-1.00026-2
Popis: A tandem mass spectrometer involves two stages of mass analysis (MS1 and MS2) separated in time or space by a mass or charge-changing reaction, most often ion dissociation. The MS2 mass spectrum of the reaction products can provide a variety of information about the ions selected by MS1. Early MS/MS instruments were based on magnetic and electric sector mass analyzers with collisional dissociation energies in the kV range. Later MS/MS instruments used two quadrupole mass analyzers separated by an radio frequency-only quadrupole reaction chamber. Efficient low-energy fragmentation, coupled with inexpensive analyzers, brought `triple quadrupole' MS/MS into wide-spread adoption. Improvements in the instrumentation, including adaptation to emerging ionization technologies and wider areas of application, have ensured its continued popularity.
Databáze: OpenAIRE
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