COMPARATIVE STUDY OF AMYLOSE AND CELLULOSE DERIVATIZED CHIRAL STATIONARY PHASES IN THE REVERSED-PHASE MODE

Autor:	Hayashi Matsukura, Hirofumi Kawabata, Masato Kazusaki
Rok vydání:	2000
Předmět:	Chromatography Chemistry Elution Clinical Biochemistry Pharmaceutical Science Biochemistry High-performance liquid chromatography Analytical Chemistry Chiral column chromatography Impurity Phase (matter) Enantiomer Enantiomeric excess Chiral derivatizing agent
Zdroj:	Journal of Liquid Chromatography & Related Technologies. 23:2819-2828
ISSN:	1520-572X 1082-6076
DOI:	10.1081/jlc-100101234
Popis:	A direct, isocratic, and simple reversed-phase HPLC method was described for the separation of enantiomers, the newly synthesized potential drug substance and corresponding optical impurity, employing polysaccharide-based chiral stationary phases (Chiralpak AD-RH and Chiralcel OD-RH). A baseline separation was attained with both columns. These two chiral stationary phases exhibit opposite chiral discrimination patterns concerning the elution order of enantiomers. Chiralpak AD-RH is more suitable for resolving the enantiomers. Low level quantification (0.05%) of the minor enantiomer is achieved. The analytical procedure was successfully applied to the detection of the optical impurity in a potential drug substance. The minor enantiomer was not determined quantitatively because of a trace level of it in the potential drug substances.
Databáze:	OpenAIRE
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