Astragaloside IV Induces Apoptosis, G1-Phase Arrest and Inhibits Anti-apoptotic Signaling in Hepatocellular Carcinoma
| Autor: | Jing Gung Chung, Hsiao Chia Wang, Chun Min Su, Chun Hui Lu, Chien Kai Lai, Fei-Ting Hsu, Yu Cheng Kuo |
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| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
Cancer Research Cell growth business.industry medicine.medical_treatment Intrinsic apoptosis medicine.disease digestive system diseases General Biochemistry Genetics and Molecular Biology Blot 03 medical and health sciences 0302 clinical medicine Apoptosis 030220 oncology & carcinogenesis Hepatocellular carcinoma Toxicity medicine Cancer research Cytotoxicity business Adjuvant |
| Zdroj: | In Vivo. 34:631-638 |
| ISSN: | 1791-7549 0258-851X |
| DOI: | 10.21873/invivo.11817 |
| Popis: | Background/aim Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and the third leading cause of cancer death worldwide. Although multiple chemotherapies options are available for HCC, chemo-induced toxicity is inevitable during clinical treatment. Therefore, identifying possible adjuvant agents with both liver-protective and antitumor effects is critical. Herbal medicines have chemopreventive and anti-HCC effect, such as Juzen taiho-to and Sho-saiko-to. Astragaloside IV is a compound extracted from the Chinese medical herb Astragalus membranaceus (Fisch.) Bge. with liver protection potential. However, whether astragaloside IV may also possess tumor-inhibitory capability and its underlying mechanism is remaining unknown. Materials and methods Viability analysis, cell-cycle analysis, apoptosis analysis, western blotting analysis and invasion trans-well assay were performed to identify tumor-inhibitory potential of astragaloside IV on HCC cells (SK-Hep1 and Hep3B cells). Results We found that astragaloside IV may induce cytotoxicity and extrinsic/intrinsic apoptosis effect, but also trigger G1 arrest in HCC cells. The expression of anti-apoptotic proteins of HCC were all reduced by astragaloside IV. Additionally, astragaloside IV also suppressed HCC cell invasion ability. Conclusion Astragaloside IV effectively suppressed HCC cell proliferation, invasion and anti-apoptosis in vitro. |
| Databáze: | OpenAIRE |
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