Ecdysteroidostatin from the house fly,Musca domestica
| Autor: | Qian-jun Li, T.S. Adams |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Ecdysteroid animal structures Sucrose integumentary system Physiology fungi Ovary General Medicine Biology Trypsin Biochemistry chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry In vivo Insect Science Internal medicine medicine Secretion Incubation hormones hormone substitutes and hormone antagonists Ecdysteroid secretion medicine.drug |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 38:166-176 |
| ISSN: | 1520-6327 0739-4462 |
| DOI: | 10.1002/(sici)1520-6327(1998)38:4<166::aid-arch2>3.0.co;2-p |
| Popis: | Ovaries from house flies maintained on sucrose secrete large amounts of ecdysteroid when they are cultured with ovarian ecdysteroidogenic hormone, OEH. However, ovarian ecdysteroid secretion is reduced by incubation with both OEH and the ovarian ecdysteroidostatin (OES). A partially purified OES fraction from a semi-preparative reverse phase HPLC C18 column caused a 98% inhibition of ovarian ecdysteroid secretion in vitro at a concentration of 0.8 equivalents per μl. Ovaries can be activated to produce ecdysteroid in vivo by feeding diet containing protein to flies maintained on sucrose. Ecdysteroid secretion was inhibited when the in vivo stimulated ovaries were cultured with OES. This suggests that OES does not interfere with the OEH activation mechanism, but blocks ovarian ecdysteroid synthesis or release. Furthermore, OES inhibition is reversible and ecdysteroid secretion resumes when OES is removed. Musca OES could explain the decrease in ecdysteroid levels found in flies after mid-vitellogenesis. Both adult male and female abdomens contain OES, but OES was not transferred to females during mating. Evidence is presented that OES is not a trypsin modulating oostatic factor. Arch. Insect Biochem. Physiol. 38:166–176, 1998. © 1998 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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