Popis: |
Fifty-eight strains of eight Bacillus species were selected from the ARS Culture Collection to investigate their ability to bioconvert oleic acid to unique hydroxy or keto derivative. Addition of 0.5% oleic acid substrate to a whole-cell culture of Bacillus strain NRRL BD-447 produced mixtures of mono-, di-, and trisubstituted compounds. These products appear to contain combinations of keto and hydroxy groups. One of the major products was isolated by high-performance liquid chromatography and its structure was determined by gas chromatography-mass spectrometry and nuclear magnetic resonance to be 7-hydroxy-16-oxo-9-cis-octadecenoic acid. |