High Expression of Metallo-β-Lactamase Contributed to the Resistance to Carbapenem in Clinical Isolates of Pseudomonas aeruginosa from Baotou, China
| Autor: | Dong Zhang, Huimin Wang, Haiying Niu, Tongping Hu, Shanna Su, Lixia Zhang, Huijie He, Yanfeng Xu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology Imipenem Carbapenem Pseudomonas aeruginosa Point mutation 030106 microbiology biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses medicine.disease_cause Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases Antibiotic resistance polycyclic compounds medicine Pharmacology (medical) 030212 general & internal medicine Efflux Gene medicine.drug Regulator gene |
| Zdroj: | Infection and Drug Resistance. 13:35-43 |
| ISSN: | 1178-6973 |
| DOI: | 10.2147/idr.s233987 |
| Popis: | Background Bacterial resistance to antibiotics has become a major public health concern. This study aimed to determine the resistance mechanisms to carbapenem in clinical isolates of Pseudomonas aeruginosa. Methods A total of 62 clinical isolates of carbapenem-resistant P. aeruginosa (CRPA) were collected from 2015 to 2017. Imipenem (IPM)-EDTA disk synergy test was used to screen strains that produced metallo-β-lactamase. In addition, the genes for outer membrane protein OprD2, metallo-β-lactamase and mexR gene were amplified and sequenced. Expression of mexA was detected by real-time PCR. Results Disk synergy test showed that 51.6% (32/62) of the strains were positive for metallo-β-lactamase. PCR showed that 84.4% of the strains were SIM-positive (27/32), 15.6% of the strains were IMP-positive (5/32), and 12.5% of the strains were VIM-positive (4/32). SPM-positive and GIM-positive strains were not detected. In addition, 5 of the 62 strains had small deletions and/or point mutations in OprD2. Three strains had a high expression of mexA, while eight strains were positive for the regulatory gene mexR with no mutations detected by DNA sequencing. Conclusion Expression of metallo-β-lactamase is the main resistance mechanism of P. aeruginosa to carbapenem. Mutations in OprD2 and/or the overexpression of efflux pump MexAB-OprM may contribute to P. aeruginosa resistance to carbapenem. |
| Databáze: | OpenAIRE |
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