Expression and properties of human TNF peptide fragments

Autor: L. N. Shingarova, S. V. Guryanova, S. A. Yakimov, E. F. Boldyreva, Alexei N. Nekrasov, E. A. Kryukova, Mikhail P. Kirpichnikov, D. A. Dolgih, Lada E. Petrovskaya
Rok vydání: 2010
Předmět:
Zdroj: Russian Journal of Bioorganic Chemistry. 36:301-309
ISSN: 1608-330X
1068-1620
Popis: The tumor necrosis factor (TNF) is a proinflammatory cytokine that plays a pivotal role in the regulation of the human immune system. Studies of the TNF functional topography are a challenging task in bioengineering. We have produced genes encoding the peptides D1 (3–30), D2 (31–85), D3 (86–114), and D4 (115–157), which correspond to isolated fragments of the informational structure of TNF. These genes were expressed in E. coli cells at a high level in a soluble form. We have shown that hybrid proteins SD2 and SD4 tend to form high-molecular aggregates, which can be destroyed by urea treatment. Purified peptides D1, D3, and D4 possess a similar secondary structure with dominating beta-structural elements. The analysis of the biological activity of these peptides has shown that they do not exhibit cytotoxic properties on L929 murine fibroblasts. The simultaneous addition of D1 with full-length TNF results in the concentration dependent suppression of TNF activity.
Databáze: OpenAIRE
Externí odkaz: