A fourier-transform infrared spectroscopic and microscopic study of the infection of potato tubers byErwinia carotovora sspcarotovora in aerobic and anaerobic conditions
| Autor: | Derek Stewart, G D Lyon, E J B Tucker |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Nutrition and Dietetics
biology Starch food and beverages Erwinia biology.organism_classification medicine.disease Anaerobic infection Microbiology Cell wall chemistry.chemical_compound chemistry biology.protein medicine Amylase Food science Agronomy and Crop Science Anaerobic exercise Solanaceae Bacteria Food Science Biotechnology |
| Zdroj: | Journal of the Science of Food and Agriculture. 66:145-154 |
| ISSN: | 1097-0010 0022-5142 |
| DOI: | 10.1002/jsfa.2740660207 |
| Popis: | The infection or potato tubers (Solanum tuberosum) by Erwinia carotovora ssp carotovora in aerobic and anaerobic conditions was studied using chemical analysis and Fourier-transform infrared (FT-IR) microscopy and diffuse reflectance spectroscopy. Analysis or the uninfected and infected residues following sequential extraction with diethyl ether, water (40°C), 90% dimethyl sulphoxide (DMSO) and digestion with amyloglucosidase showed that the principal effect of infection, particularly in anaerobic conditions, is starch degradation. This was substantiated by gel filtration chromatography of the 90% DMSO extracts. Extensive lignification was not found to be intimately associated with either infective condition. FT-IR microscopical analysis of tissue sections showed that infection in both aerobic and anaerobic conditions caused starch and cell wall degradation, whilst extensive membrane degradation was only evident with anaerobic infection. |
| Databáze: | OpenAIRE |
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