Homologous recombination of SV4O DNA in COS7 cells occurs with high frequency m a gene dose independent fashion

Autor:	A. Grässmann, W. Rommerskirch, M. Grässmann, I. Graeber
Rok vydání:	1988
Předmět:	biology DNA repair Base pair DNA polymerase DNA polymerase II Circular bacterial chromosome Genetics biology.protein Molecular biology Replication protein A DNA polymerase mu In vitro recombination
Zdroj:	Nucleic Acids Research. 16:941-952
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/16.3.941
Popis:	Homologous recombination between microinjected SV40 DNA fragments and endogenous SV40 DNA in COS7 cells was analysed by immunofluorescence staining and DNA blotting. Time course experiments revealed that recombination between the transferred (trans) DNA and the chromosomal DNA occurred about 8 hours after microinjection with high efficiency in a gene dose independent fashion. Deletions of up to 1018 basepairs (bp) within the early or the late SV40 region were efficiently repaired after the transfer of linear but not of circular DNA molecules. A 22 bp homology between the trans DNA and the endogenous DNA was sufficient to initiate recombination but 14 nonhomologous bp at one open end of the SV40 DNA fragments hindered gap repair.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::08fd37590a808e66cb2fa9c919a3f2e6 https://doi.org/10.1093/nar/16.3.941 Zobrazit plný text záznamu