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느타리버섯(Pleurotus ostreatus), 큰느타리버섯(Pleurotus eryngii), 팽이버섯(Flammulina velvtipes)과 노루궁뎅이버섯(Hericium erinaceum), 느티만가닥버섯(Lyophyllum ulmarium), 노랑느타리버섯(Pleurotus cornucopiae), 잣버섯(Lentinus lepideus), 버들송이버섯(Agrocybe cylindracea)의 수확 후 배지 (spent mushroom compost, SMC)로부터 ${\alpha}$ -amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$ -xylosidase (EC 3.2.1.37), ${\beta}$ -glucosidase (EC 3.2.1.21) 및 cellulase (EC 3.2.1.4)의 활성을 추출 buffer별로 조사하였다. 물과 0.25% Triton X-100가 효소 회수율이 높았으며 xylanase는 팽이버섯 SMC에서 153 U/g으로 가장 많이 생산되었으며 laccase는 큰느타리버섯 SMC에서 8.0 U/g로 가장 높았다. ${\alpha}$ -amylase (3.6 U/g)와 cellulase (3.2 U/g)는 버들송이 SMC에서 가장 많이 생산되었다. 큰느타리버섯 SMC 추출물의 laccase와 cellulase 활성은 상용 효소와 필적하는 효소활성을 보여 산업적 이용 가능성을 시사하였다. 【Recovery of ${\alpha}$ -amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), ${\beta}$ -xylosidase (EC 3.2.1.37), ${\beta}$ -glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) from spent mushroom composts (SMCs) of Pleurotus cornucopiae, Pleurotus ostreatus, Pleurotus eryngii, Hericium erinaceum, Lyophyllum ulmarium, Agrocybe cylindracea, Lentinus lepideus, and Flammulina velvtipes were investigated using different extraction buffers. The maximum recovery of the enzymes was mostly detected in SMC extracts with tap water and 0.25% Triton X-100 by shaking incubation (200 rpm) for 2 h at $4^{\circ}C$ . The xylanase (152 U/g) and laccase (8.1 U/g) activities were the highest in SMC extracts from F. velvtipes and P. eryngii. In addition, high enzymatic activities of ${\alpha}$ -amylase (3.6 U/g) and cellulase (3.4 U/g) was detected in SMC extract of A. cylindracea. Futhermore, cellulase and laccase activities of SMCE from P. eryngii were compared to commercial enzymes.】 |
