| Autor: |
Julien Fierling, Alphy John, Barthélémy Delorme, Alexandre Torzynski, Guy Blanchard, Claire Lye, Grégoire Malandain, Bénédicte Sanson, Jocelyn Étienne, Philippe Marmottant, Catherine Quilliet, Matteo Rauzi |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.1101/2021.12.14.472566 |
| Popis: |
Cell apical constriction driven by actomyosin contraction forces is a conserved mechanism during tissue folding in embryo development. While much effort has been made to better understand the molecular mechanisms responsible for apical constriction, it is still not clear if apical actomyosin contraction forces are necessary or sufficient per se to drive tissue folding. To tackle this question, we use the Drosophila embryo model system that forms a furrow on the ventral side, initiating mesoderm internalization. Past computational models support the idea that cell apical contraction forces may not be sufficient and that active or passive cell apico-basal forces may be necessary to drive cell wedging and tissue furrowing. By using 3D computational modelling and in toto embryo image analysis and manipulation, we now challenge this idea and show that embryo-scale force balance of the tissue surface, rather than cell-autonomous shape changes, is necessary and sufficient to drive a buckling of the epithelial surface forming a furrow which propagates and initiates embryo gastrulation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
