| Autor: | Naoko Norioka, Yumi Yoshimura, Takanori Matsuura, Niranjali Gamage, Takeshi Takasaki, Tetsu Nakanishi, Shigemi Norioka |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
chemistry.chemical_classification
Expression vector biology Bioengineering General Medicine medicine.disease_cause Applied Microbiology and Biotechnology Molecular biology Fusion protein Gene product Enzyme Glutathione S-transferase chemistry Gene expression biology.protein medicine Ribonuclease Escherichia coli Biotechnology |
| Zdroj: | Biotechnology Letters. 22:1413-1417 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1005617000553 |
| Popis: | S-RNase is a style-specific ribonuclease which is associated with gametophytic self-incompatibility. An expression vector of a fusion protein of Pyrus pyrifolia(Japanese pear) S3-RNase with glutathione-S-transferase (GST) was constructed and transformed into E. coli. Using this system, the fusion protein, GST-S3-RNase, was expressed as an active form and can be used for screening pollen S-gene product(s). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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