Viscum album preparations inhibit DNA methylation by preventing DNA methyltransferase-induced methyl group transfer

Autor:	J. Defize, A. Zwiers, Aldur W. Eriksson, G. Pals, J.B.A. Kipp
Rok vydání:	1994
Předmět:	Pharmacology Reporter gene Methyltransferase biology Promoter Methylation biology.organism_classification Molecular biology DNA methyltransferase chemistry.chemical_compound Complementary and alternative medicine chemistry DNA methylation Viscum album DNA
Zdroj:	British Homeopathic Journal. 83:205-208
ISSN:	0007-0785
DOI:	10.1016/s0007-0785(05)80793-8
Popis:	Interactions between Viscum album (Iscador) and DNA were studied by amplification of specific human pepsinogen A gene promoter sequences by polymerase chain reaction (PCR). Gel retardation assays showed no binding of Viscum album to these promoter sequences. Incubation of plasmid constructs consisting of human pepsigen A promoter fragments coupled to the chloramphenicol acetyltransferase (CAT) reporter gene with Iscador had no effect on transcriptional activity. Promoter sequences incubated with Iscador became insensitive to methylation by specific DNA methyltransferases by destruction of DNA methyltransferase activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::08b601c56cf324f920bbe365368648cd https://doi.org/10.1016/s0007-0785(05)80793-8 Zobrazit plný text záznamu