Sandwich ELISA for glutathione S-transferase Alpha 1-1: plasma concentrations in controls and in patients with gastrointestinal disorders
| Autor: | D. A. Court, Wilbert H.M. Peters, J. B. M. J. Jansen, Theo P.J. Mulder |
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| Rok vydání: | 1996 |
| Předmět: |
Antiserum
medicine.medical_specialty medicine.drug_class Biochemistry (medical) Clinical Biochemistry Glutathione Biology medicine.disease Monoclonal antibody Inflammatory bowel disease chemistry.chemical_compound Endocrinology chemistry Internal medicine Blood plasma Immunology medicine biology.protein Pancreatitis Antibody Glutathione S-Transferase Alpha |
| Zdroj: | Clinical Chemistry. 42:416-419 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/42.3.416 |
| Popis: | Class Alpha glutathione S-transferases (GST-Alpha) are found in high concentrations in human liver. Increased plasma concentrations of GSTA1-1, the most abundant isoform of GST-Alpha, are a very sensitive marker for hepatocellular leakage. A sandwich-type ELISA was developed, based on a monoclonal antibody specific for human GSTA1-1 and a polyclonal rabbit anti-human GST-Alpha antiserum. The assay is specific for human GSTA1-1, and has a detection limit of 0.04 micrograms/L. The distribution of plasma GSTA1-1 concentrations in 350 blood donors was nearly normalized by logarithmic transformation and an upper normal reference concentration of 5.9 micrograms/L was calculated. Men had significantly higher plasma GSTA1-1 concentrations than women (P |
| Databáze: | OpenAIRE |
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