Transcription factor Hoxb5 reprograms B cells into functional T lymphocytes

Autor: Peiqing Zhou, Qitong Weng, Sheng Xu, Qianhao Zhao, Yong-Guang Yang, Aibin He, Yong Dong, Xiaofei Liu, Lijuan Liu, Fangxiao Hu, Juan Du, Rongqun Guo, Ying Wang, Yuxian Guan, Yang Geng, Demin Wang, Jiekai Chen, Hongling Wu, Dan Yang, Bing Liu, Mengyun Zhang, Zheng Hu, Jinyong Wang, Chengxiang Xia, Tongjie Wang, Chen Li, Cui Lv
Rok vydání: 2018
Předmět:
Zdroj: Nature Immunology. 19:279-290
ISSN: 1529-2916
1529-2908
DOI: 10.1038/s41590-018-0046-x
Popis: Deletion of master regulators of the B cell lineage reprograms B cells into T cells. Here we found that the transcription factor Hoxb5, which is expressed in uncommitted hematopoietic progenitor cells but is not present in cells committed to the B cell or T cell lineage, was able to reprogram pro-pre-B cells into functional early T cell lineage progenitors. This reprogramming started in the bone marrow and was completed in the thymus and gave rise to T lymphocytes with transcriptomes, hierarchical differentiation, tissue distribution and immunological functions that closely resembled those of their natural counterparts. Hoxb5 repressed B cell ‘master genes’, activated regulators of T cells and regulated crucial chromatin modifiers in pro-pre-B cells and ultimately drove the B cell fate–to–T cell fate conversion. Our results provide a de novo paradigm for the generation of functional T cells through reprogramming in vivo. Wang and colleagues show that the transcription factor Hoxb5, which is expressed in uncommitted hematopoietic progenitor cells but is absent from committed B cells and T cells, can reprogram pro-pre-B cells into functional early T cell lineage progenitors.
Databáze: OpenAIRE
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