| Autor: | Takashi Nakamura, Koji Yakabi, Yoichi Uehigashi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Physiology medicine.medical_treatment Gastroenterology Prostaglandin Enteroendocrine cell Endogeny Mast cell Cytoprotection chemistry.chemical_compound Endocrinology medicine.anatomical_structure chemistry Internal medicine Dispase medicine Prostaglandin E2 medicine.drug Prostaglandin E |
| Zdroj: | Digestive Diseases and Sciences. 44:1384-1389 |
| ISSN: | 0163-2116 |
| DOI: | 10.1023/a:1026699718316 |
| Popis: | Endogenous prostaglandin E2 has beenindicated to have an important role in preventinggastric mucosal damage from noxious agents (ie, inadaptive cytoprotection). However, the response ofendogenous prostaglandin E2 to a mild irritant iscontroversial. In this study, we attempted to determinewhether pretreatment with a low concentration of ethanolcould induce endogenous prostaglandin E2production by isolated canine fundic mucosal cells and to identifycells that are responsible for an increase ofprostaglandin E production. Canine fundic mucosa wasdigested by collagenase, dispase, and EDTA. The cellswere separated into five fractions with anelutriator rotor. Pretreatment with 5% ethanol induceda significant increase of prostagladin E2release only from the secondary small-sized cellfraction, which was rich in mast cells and endocrine cells, and notfrom the other four fractions. Further cell separationby density gradient centrifugation revealed that themast cell-enriched fraction (54%) was responsible for the increase of prostaglandin E2release induced by the pretreatment with 5% ethanol. Theresults suggest that mast cells of the gastric mucosaplay an important role in the production of endogenous prostaglandin E2 in adaptivecytoprotection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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