Development and Validation of Analytical Methods for the Detection and Quantification of a Novel Dimeric Ceramide in Stratum Corneum and Other Layers of the Skin
| Autor: | Bodo Dobner, Reinhard H.H. Neubert, Sophie C Steinbach, Olena Bayrak, Stefan Sonnenberger |
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| Rok vydání: | 2016 |
| Předmět: |
Detection limit
Lc apci ms Ceramide Complex matrix Chromatography integumentary system 010401 analytical chemistry Organic Chemistry Clinical Biochemistry 01 natural sciences Biochemistry Dosage form 0104 chemical sciences Analytical Chemistry 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure chemistry Stratum corneum medicine Barrier function |
| Zdroj: | Chromatographia. 79:1615-1624 |
| ISSN: | 1612-1112 0009-5893 |
| Popis: | Ceramides (CERs) are integral parts of the intercellular lipid lamellae of the stratum corneum (SC), which is responsible for the barrier function of the skin. Many skin diseases such as atopic dermatitis and psoriasis are associated with depletion or disturbance of the level of CERs in the SC. Administration of an exogenous novel dimeric ceramide (dCER) deep into the SC may help to stabilize the SC barrier substantially and to treat some skin disease conditions and with the help of the existing technology it might be possible to formulate various pharmaceutical dosage forms that can facilitate penetration of dCER into the SC. However, assessment of the rate and extent of permeability of the exogenous dCER involves appropriate analytical techniques which can discriminately quantify the exogenous dCER in the SC and in the other skin layers. Therefore, an attempt was made to develop an AMD-HPTLC and an HPLC/APCI-MS method for the detection and quantification of exogenous dCER in the SC as well as other skin layers. The method involved synthesis of the dCER and development of appropriate HPTLC and LC/ESI-MS methods for the separation and quantification of the dCER in the SC and deeper layers of the skin. The methods developed were optimized for quantification of a novel dCER. In comparison to the AMD-HPTLC method, the HPLC/MS method offers a higher sensitivity. Both methods could be used for the quantification of dCER in presence of a complex matrix (e.g., skin extract). The developed methods are complementary and could be used for the quantification of dCER in any further stage of substance research and industrial application. The methods developed are robust, linear and sensitive with a low limit of detection (LOD) and low limit of quantification (LOQ). |
| Databáze: | OpenAIRE |
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