Isolation and characterization of microsatellite loci in Melicope quadrilocularis (Rutaceae), an endemic plant species of the Bonin Islands, Japan, and cross-species amplification in closely related taxa

Autor: Saeko Katoh, Yuji Isagi, Noriaki Murakami, Hidetoshi Kato, Shingo Kaneko
Rok vydání: 2007
Předmět:
Zdroj: Conservation Genetics. 8:1487-1490
ISSN: 1572-9737
1566-0621
DOI: 10.1007/s10592-007-9304-4
Popis: Twelve microsatellite loci were isolated and characterized for Melicope quadrilocularis, an insular endemic tree species of the Bonin Islands. The observed number of alleles at each locus ranged from 1 to 18. The range of expected heterozygosity was 0.0000–0.9445. The inter-specific applicability of these loci was evaluated by analyzing two other endemic species and one endemic variety of Melicope that are also distributed on the Bonin Islands. All primer pairs for the 12 loci tested successfully amplified the loci in all taxa, except for primers for four loci in M. nishimurae.
Databáze: OpenAIRE