Cloning, Expression, and Purification of a Pathogenesis-Related Protein from Oenanthe javanica and Its Biological Properties
| Autor: | Jeong Won Ahn, Bo Ram Jo, Hyun Soo Kim, Deuk Hee Jin, Su-Kil Jang, Eun A Seoung, Jung Min Yu, Seong Soo Joo, Hee Yong Park |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pharmacology Chemokine biology Chemistry Microarray analysis techniques Pharmaceutical Science General Medicine Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine High-mobility group Interferon 030220 oncology & carcinogenesis TLR4 biology.protein medicine Signal transduction Protein kinase A medicine.drug Pathogenesis-related protein |
| Zdroj: | Biological and Pharmaceutical Bulletin. 43:158-168 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b19-00801 |
| Popis: | Pathogenesis-related (PR) proteins are inducible and accumulated in plants upon pathogen challenge for survival. Interest in these proteins has arisen in many fields of research, including areas of protein defense mechanisms and plant-derived allergens. In this study, we cloned a PR protein gene (OJPR) from Oenanthe javanica, which consisted of 465 bp with an approximate molecular mass of 16 kDa. The DNA and deduced amino acid sequences of OJPR were 87% similar to Pimpinella brachycarpa PR-1 together with a glycine-rich loop which is a signature motif of PR-10. In microarray analysis, OJPR-transfected Raw264.7 (OJPR+) upregulated high mobility group box 1 and protein kinase Cα, and downregulated chemokine ligand 3 and interleukin 1β which are all related to toll-like receptor 4 (TLR4) and inflammation. TAK-242 and PD98059 inhibited the activation by OJPR, suggesting that OJPR transduce TLR4-mediated signaling. Interestingly, OJPR increased anti-viral repertoires, including interferon (IFN)α, IFNγ, OAS1, and Mx1 in CD4+ primary T cells. Taken together, we concluded that OJPR may play a role in modulating host defense responses via TLR signal transduction and provide new insights into the therapeutic and diagnostic advantages as a potential bioactive protein. |
| Databáze: | OpenAIRE |
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