Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
Autor: | Elaine C. Johnson, Hari Jayaram, Diana C Lozano, Dongseok Choi, John C. Morrison |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
genetic structures Microarray Optic disk RNA RNA-Seq Computational biology Biology eye diseases Gene expression profiling 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Gene expression 030221 ophthalmology & optometry Optic nerve sense organs DNA microarray |
Zdroj: | Glaucoma ISBN: 9781493974061 |
DOI: | 10.1007/978-1-4939-7407-8_20 |
Popis: | Understanding the cellular pathways activated by elevated intraocular pressure (IOP) is crucial for the development of more effective glaucoma treatments. Microarray studies have previously been used to identify several key gene expression changes in early and extensively injured ONH, as well as in the retina. Limitations of microarrays include that they can only be used to detect transcripts that correspond to existing genomic sequencing information and their narrower dynamic range. However, RNA sequencing (RNA-seq) is a powerful tool for investigating known transcripts, as well as for exploring new ones (including noncoding RNAs and small RNAs), is more quantitative, and has the added benefit that the data can be re-analyzed as new sequencing information becomes available. Here, we describe an RNA-seq method specifically developed for identifying differentially expressed genes in optic nerve heads of eyes exposed to elevated intraocular pressure. The methods described here could also be applied to small tissue samples (less than 100 ng in total RNA yield) from retina, optic nerve, or other regions of the central nervous system. |
Databáze: | OpenAIRE |
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