| Popis: |
Summary Sedimentation analytically and gelchromatographically homogeneous 11S globulin from sunflower seed was negatively stained with different anionic or cationic staining agents, fixed and studied electron microscopically. The globulin molecule, the molecular weight of which is about 300,000 g/mol, has outer dimensions of 10.4 nm × 10.4 nm × 8.8 nm. For the quaternary structure of the molecule a model is proposed consisting of six spherical subunits, which are arranged in, form of a trigonal antiprism with the point symmetry 32. The subunits have a central channel parallel to the 3-fold molecule axis. The axial ratio of the molecule is 0.85 and the hydration 0.25 g solvent/g protein. |
