Swarming of Pseudomonas aeruginosa PAO1 without differentiation into elongated hyperflagellates on hard agar minimal medium
| Autor: | Yoji Nakagawa, Jun-ichi Wakita, Mitsugu Matsushita, Takashi Nozawa, Tohey Matsuyama, Taichiro Tanikawa, Chihiro Takahashi |
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| Rok vydání: | 2008 |
| Předmět: |
food.ingredient
Pseudomonas aeruginosa Rhamnolipid biochemical phenomena metabolism and nutrition Biology Flagellum biology.organism_classification medicine.disease_cause Microbiology Staining Agar plate Complementation chemistry.chemical_compound food chemistry Genetics medicine bacteria Agar Flagellate Molecular Biology |
| Zdroj: | FEMS Microbiology Letters. 280:169-175 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2007.01057.x |
| Popis: | Polar flagellated Pseudomonas aeruginosa PAO1 demonstrated extensive spreading growth in 2 days on 1.5% agar medium. Such spreading growth of P. aeruginosa PAO1 strains was absent on Luria-Bertani 1.5% agar medium, but remarkable on Davis minimal synthetic agar medium (especially that containing 0.8% sodium citrate and 1.5% Eiken agar) under aerobic 37 degrees C conditions. Analyses using isogenic mutants and complementation transformants showed that bacterial flagella and rhamnolipid contributed to the surface-spreading behavior. On the other hand, a type IV pilus-deficient pilA mutant did not lose the spreading growth activity. Flagella staining of PAO1 T cells from the frontal edge of a spreading colony showed unipolar and normal-sized rods with one or two flagella. Thus, the polar flagellate P. aeruginosa PAO1 T appears to swarm on high-agar medium by producing biosurfactant rhamnolipid and without differentiation into an elongated peritrichous hyperflagellate. |
| Databáze: | OpenAIRE |
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