Whole Mount In Situ Hybridization in Zebrafish v1

Autor:	D R Hammond-Weinberger
Rok vydání:	2022
DOI:	10.17504/protocols.io.b74vrqw6
Popis:	Whole mount in situ hybridization protocol optimized for single gene detection using chromogenic substrates NBT/BCIP in zebrafish (Danio rerio). Options are included for bleaching and permeabilization. This protocol beings with tissue preparation and ends with glycerol mounting for imaging. Probe synthesis is not included. This protocol has been used for embryos/larvae from 24 - 72 hpf.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::08244d3647387055df354318c98295f8 https://doi.org/10.17504/protocols.io.b74vrqw6 Zobrazit plný text záznamu