Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin
Autor: | Manoj G Pillai, Ravi Sarada, Gokare Aswathanarayana Ravishankar |
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Rok vydání: | 1999 |
Předmět: |
Spirulina (genus)
Chromatography biology Chemistry Extraction (chemistry) Biomass Bioengineering Water extraction biology.organism_classification Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Pigment Phycocyanobilin Leaching (chemistry) visual_art Phycocyanin visual_art.visual_art_medium |
Zdroj: | Process Biochemistry. 34:795-801 |
ISSN: | 1359-5113 |
DOI: | 10.1016/s0032-9592(98)00153-8 |
Popis: | A number of drying methods studied for the processing of Spirulina (crossflow dried, spray dried and oven dried) resulted in approximately 50% loss of phycocyanin. Therefore fresh biomass was suitable for phycocyanin extraction. Of the extraction methods tested, freezing and thawing of cells, homogenisation using a mortar and pestle in the presence of abrasive material and homogenisation using a blender at 10 000 rpm yielded 19.4±0.4 mg phycocyanin per 100 mg dry weight of Spirulina while water extraction was a slow process. Acid treatment also resulted in phycocyanin leaching. Phycocyanin was stable over a pH range of 5–7.5 at 9±1°C, whereas temperature beyond 40°C lead to instability. The pigment phycocyanobilin was separated from the phycocyanin. |
Databáze: | OpenAIRE |
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