Expression of Green Fluorescent Protein in Aureobasidium pullulans and Quantification of the Fungus on Leaf Surfaces
| Autor: | J H Andrews, B Schoenike, Daniel Cullen, A Vanden Wymelenberg, R N Spear |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Reporter gene
biology fungi Fungi imperfecti biology.organism_classification Fluorescence General Biochemistry Genetics and Molecular Biology Green fluorescent protein law.invention Microbiology Aureobasidium pullulans Transformation (genetics) Biochemistry Confocal microscopy law Gene expression Biotechnology |
| Zdroj: | BioTechniques. 23:686-690 |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/97234st01 |
| Popis: | A red-shifted, mutated form of the jellyfish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus, including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intracellular distribution of GFP was nonuniform. When applied to leaf surfaces, the transformants were readily visible and amenable to quantification by image analysis. Thus, GFP expression, together with quantitative image analysis, may provide a powerful method for ecological studies of plant-microbe relationships in nature. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|