Drug-induced interference in fluorometric histamine assays
| Autor: | M. Schnabel, Wilfried Lorenz, Madeleine Ennis |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pharmacology
Drug Allergy Radiographic contrast media Demeclocycline media_common.quotation_subject Immunology Toxicology medicine.disease Mast cell chemistry.chemical_compound Procaine medicine.anatomical_structure chemistry medicine Pharmacology (medical) Rolitetracycline Histamine medicine.drug media_common |
| Zdroj: | Agents and Actions. 36:C177-C180 |
| ISSN: | 1420-908X 0065-4299 |
| Popis: | Although drug-induced interferences have been widely discussed for plasma histamine assays, they are often neglected in reports using isolated mast cells. This study examined the effect of thirty-three different drugs on the two most usual histamine assays used for histamine release experiments with mast cells, namely the fluorometric condensation assay usingo-phthaldialdehyde with and without solvent extraction steps. Solvent extraction was required in order to assay histamine in the presence of 13 of the tested drugs. These included all radiographic contrast media tested and several antibiotics. Even after solvent extraction 3 drugs, demeclocycline, procaine and rolitetracycline, interfered with the histamine assay. Thus, even at relatively high histamine concentrations, as are realised in rat peritoneal mast cell experiments, drugs may cause interfering effects in assay results. Investigations of the signals given by the drug alone and in the presence of suitable histamine standards should be mandatory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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